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sx4250 rotor  (Beckman Coulter)


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    Structured Review

    Beckman Coulter sx4250 rotor
    Sx4250 Rotor, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sx4250+rotor/SX4250+Swinging-Bucket+Rotor/pmc07295053-54-11-13
    Average 93 stars, based on 61 article reviews
    sx4250 rotor - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection
    Article Snippet: .. With subsequent centrifugation at 800 g for 20 min at RT in a Beckman SX4250 rotor without brake, the mononuclear leukocytes were collected at the gradient interface, washed in PBS, and resuspended in flow cytometry buffer containing 1% bovine serum albumin and 0.02% sodium azide in PBS. .. BILs were incubated with 0.5 μg Fc block (anti-FcyRIII/II mAb) prepared from the supernatant of 2.4G2 hybridoma cells for 30 min on ice, followed by staining with CD45 (clone 30-F11, BD Biosciences), CD11b (clone M1/70, BD Biosciences), Ly6G/C (clone RB6-8C5, BD Biosciences), or Ly6G (clone 1A8, BD Biosciences).

    Article Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection
    Article Snippet: .. With subsequent centrifugation at 800 g for 20 min at RT in a Beckman SX4250 rotor without brake, the mononuclear leukocytes were collected at the gradient interface, washed in PBS, and resuspended in flow cytometry buffer containing 1% bovine serum albumin and 0.02% sodium azide in PBS. .. BILs were incubated with 0.5 μg Fc block (anti-FcyRIII/II mAb) prepared from the supernatant of 2.4G2 hybridoma cells for 30 min on ice, followed by staining with CD45 (clone 30-F11, BD Biosciences), CD11b (clone M1/70, BD Biosciences), Ly6G/C (clone RB6-8C5, BD Biosciences), or Ly6G (clone 1A8, BD Biosciences).

    Article Title: Purification, crystallization and preliminary crystallographic analysis of the CBS-domain protein MJ1004 from Methanocaldococcus jannaschii
    Article Snippet: The fractions of interest were combined and concentrated using Vivaspin centrifugal concentrators (5000 molecular-weight cutoff) to a volume of approximately 0.5 ml and injected onto a HiLoad Superdex 75 16/60 Prep Grade column (GE Healthcare). .. The E. coli proteins precipitated by the heat shock were removed by centrifugation at 4000 g in an SX4250 rotor (Beckman) for 15 min at 277 K. The supernatant was filtered through a 0.22 µm filter and then injected onto a 5 ml HiTrap SP column (GE Healthcare) at a flow rate of 1 ml min −1 , washed with several column volumes of buffer A (50 m M HEPES pH 7.0, 1 m M EDTA, 1 m M β-mercaptoethanol) and eluted with a stepped gradient of buffer B (50 m M HEPES pH 7.0, 1 M NaCl, 1 m M EDTA, 1 m M β-mercaptoethanol) over 30 min. .. The fractions of interest were combined and concentrated using Vivaspin centrifugal concentrators (5000 molecular-weight cutoff) to a volume of approximately 0.5 ml and injected onto a HiLoad Superdex 75 16/60 Prep Grade column (GE Healthcare).

    Flow Cytometry:

    Article Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection
    Article Snippet: .. With subsequent centrifugation at 800 g for 20 min at RT in a Beckman SX4250 rotor without brake, the mononuclear leukocytes were collected at the gradient interface, washed in PBS, and resuspended in flow cytometry buffer containing 1% bovine serum albumin and 0.02% sodium azide in PBS. .. BILs were incubated with 0.5 μg Fc block (anti-FcyRIII/II mAb) prepared from the supernatant of 2.4G2 hybridoma cells for 30 min on ice, followed by staining with CD45 (clone 30-F11, BD Biosciences), CD11b (clone M1/70, BD Biosciences), Ly6G/C (clone RB6-8C5, BD Biosciences), or Ly6G (clone 1A8, BD Biosciences).

    Article Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection
    Article Snippet: .. With subsequent centrifugation at 800 g for 20 min at RT in a Beckman SX4250 rotor without brake, the mononuclear leukocytes were collected at the gradient interface, washed in PBS, and resuspended in flow cytometry buffer containing 1% bovine serum albumin and 0.02% sodium azide in PBS. .. BILs were incubated with 0.5 μg Fc block (anti-FcyRIII/II mAb) prepared from the supernatant of 2.4G2 hybridoma cells for 30 min on ice, followed by staining with CD45 (clone 30-F11, BD Biosciences), CD11b (clone M1/70, BD Biosciences), Ly6G/C (clone RB6-8C5, BD Biosciences), or Ly6G (clone 1A8, BD Biosciences).

    Injection:

    Article Title: Purification, crystallization and preliminary crystallographic analysis of the CBS-domain protein MJ1004 from Methanocaldococcus jannaschii
    Article Snippet: The fractions of interest were combined and concentrated using Vivaspin centrifugal concentrators (5000 molecular-weight cutoff) to a volume of approximately 0.5 ml and injected onto a HiLoad Superdex 75 16/60 Prep Grade column (GE Healthcare). .. The E. coli proteins precipitated by the heat shock were removed by centrifugation at 4000 g in an SX4250 rotor (Beckman) for 15 min at 277 K. The supernatant was filtered through a 0.22 µm filter and then injected onto a 5 ml HiTrap SP column (GE Healthcare) at a flow rate of 1 ml min −1 , washed with several column volumes of buffer A (50 m M HEPES pH 7.0, 1 m M EDTA, 1 m M β-mercaptoethanol) and eluted with a stepped gradient of buffer B (50 m M HEPES pH 7.0, 1 M NaCl, 1 m M EDTA, 1 m M β-mercaptoethanol) over 30 min. .. The fractions of interest were combined and concentrated using Vivaspin centrifugal concentrators (5000 molecular-weight cutoff) to a volume of approximately 0.5 ml and injected onto a HiLoad Superdex 75 16/60 Prep Grade column (GE Healthcare).



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